Fig. 4.
Expression of CD22ΔE12 transcriptome in relapsed BPL cells.
The 43-gene mouse CD22ΔE12-Tg transcriptome was represented by 110 probesets on the human U133 Plus 2.0 Array. The RMA-normalized gene expression values for leukemia cells from 49 BPL patients in relapse (GSE28460) were log2 transformed and mean-centered to the average value for the 447 diagnosis samples (GSE11877, GSE13351, GSE28460, and GSE7440). Mixed model ANOVA showed significant overall increase in expression of 110 probesets in relapsed cases (F1,494 = 68.7, P < 0.0001). (A) The gene expression values were clustered according to average distance metric. Heat map depicts up- and downregulated transcripts ranging from red to green respectively for expression values mean centered to diagnosis samples. (B) To determine the differential expression of each leading edge gene of the CD22ΔE12 transcriptome in relapsed BPL cells, linear contrasts were performed for the RMA normalized values (P < 0.05 deemed significant). 33 probesets were upregulated and 5 downregulated with P < 0.0001 and fold change greater than 2.