Table 2.

Quantitative real-time PCR test results for a Gallus gallus domesticus gDNA dilution series over five orders of magnitude (10⁴–10⁰) for the amplification of cytoplasmic beta-actin (actb), beta-2 microglobulin (β₂m), glyceraldehyde 3-phosphate dehydrogenase (gapdh), and tata-binding protein (tbp) protein-encoding gene fragments. Mean and standard deviation are provided for the coefficient of determination, slope, amplification factor and efficiency of three replicate reactions targeting the four loci.

				Coefficient of determination (R2)		Slope		Amplification factor		Efficiency (%)
Locus	Repeat	Template	Linear range	Value	Mean ± SD	Value	Mean ± SD	Value	Mean ± SD	Value	Mean ± SD
actb	1	Genomic DNA	1.05–1.00	0.990	0.985 ± 0.009	− 2.920	− 3.048 ± 0.198	2.253	2.124 ± 0.119	120.022	113.452 ± 9.992
	2	Genomic DNA	1.04–1.00	0.990		− 3.276		2.020		101.953
	3	Genomic DNA	1.04–1.00	0.975		− 2.948		2.100		118.380
β2m	1	Genomic DNA	1.05–1.00	0.974	0.983 ± 0.009	− 2.938	− 3.066 ± 0.270	2.185	2.121 ± 0.123	118.961	112.984 ± 13.256
	2	Genomic DNA	1.04–1.00	0.984		− 3.376		1.979		97.792
	3	Genomic DNA	1.04–1.00	0.992		− 2.884		2.200		122.198
gapdh	1	Genomic DNA	1.05–1.00	0.978	0.975 ± 0.009	− 1.910	− 2.400 ± 0.427	3.300	2.685 ± 0.533	233.857	170.529 ± 54.955
	2	Genomic DNA	1.04–1.00	0.965		− 2.690		2.354		135.368
	3	Genomic DNA	1.04–1.00	0.983		− 2.601		2.400		142.364
tbp	1	Genomic DNA	1.05–1.00	0.992	0.988 ± 0.006	− 2.867	− 3.053 ± 0.205	2.247	2.137 ± 0.114	123.253	113.247 ± 10.632
	2	Genomic DNA	1.04–1.00	0.981		− 3.019		2.144		114.405
	3	Genomic DNA	1.04–1.00	0.992		− 3.273		2.020		102.083