Fig. 2.

xWORM optimization of the schistosome egg hatch assay. Background motility index measurements over time at frequencies of 10, 25 and 50 KHz, generated from xCELLigence wells with 200 μl 0.1× PBS without parasites (Panel A). Egg motility index curves blanked against 0.1× PBS alone controls (Panel B). Total motility index over 16 h (Panel C). Strictly standardized mean difference (SSMD) for the three frequencies (Panel D). An SSMD′ score above 7 indicates an “excellent assay”, and a treatment SSMD score above 1.645 is equivalent obtains a significance level of P ≤ 0.05 and represents a positive hit. For all panels: the mean of biological duplicate experiments is plotted with standard error of the mean (SEM) error bars.