Figure 1. 1,25D suppresses expression of different subunits of NF-κB protein.

(a) Suppression of NF-κB protein expression in HT29 cells by 1,25D (100 nM) treatment. (b) Quantification of western blots of NF-κB expression in 1,25D treated HT29 cells from three different experiments; NF-κB1, NF-κB2, and RELA respectively. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with paired control samples. (c) 1,25D suppresses expression of different subunits of NF-κB protein in SCC25 HNSCC cells. Note that the P100/P52 blot was stripped and reprobed for P65. (d) NF-κB mRNA expression after 1,25D treatment in HT29, SCC25 and LNCaP cells, as indicated.