Figure 2. Receptor binding avidity of NC/02, NC/02^HA149, and TN/09 influenza viruses.

Each virus binding to sialylglycopolymers containing either α2,3- or α2,6-sialylated was measured by solid-phase direct binding assays (a) and dose-dependent direct glycan binding assays (b). Avidity assays were confirmed by performing a standard hemagglutinin assay with the resialylated cRBCs; untreated cRBCs (upper), VCNA-treated cRBCs (middle), or α2,6-resialylated cRBCs (bottom) (c). The images are representatives from four independent experiments. In each assay described NC/02^HA149 bound more strongly to the α2,6 receptor.