Fig. 4.

Pectinase fragments amplified from S. levis cDNA and genomic DNA. PCR conducted with primers used for cloning ORFs in pPICZα vectors and primers for qRT-PCR analysis. (A) Representative scheme of primer position on coding sequence of mature Sl-pectinases. (B) Gel separation of fragments generated by PCR; M, GeneRuler ladder 1 kb (Thermo Scientific Fermentas, CA); 1–4, primer combinations; I, reaction with genomic DNA template; II, reaction with cDNA template; III, negative control. (C) Alignment between Sl-endoPG sequences from both genomic and cDNA samples, showing two small introns. Sequences underlined in white indicate forward and reverse primers used for sequencing of genomic Sl-endoPG. Asterisks indicate 5′ and 3′ of two introns identified.