Fig. 5.

Grhl genes are expressed in the prospective SE. (A–N) In situ hybridization of whole-mount embryos (A–D, H–K), sagittal sections (E, L), and transverse sections (F, G, M, N) at E7.25 (A, H), E7.5 (B, I), and E7.8 (C–G, J–N). Lateral view (A–C, H–J), frontal view (D), and caudal view (K). (O-a) Whole-mount immunofluorescent staining (O–V, Y-a) and sectioning image (X) at E8.0 (O, P, R), E8.25 (S, U, Y), and E8.5 (Q, T, V, Z). (W) A section plane (X) is indicated in the schematic. Expression of GRHL2 (O–Q: GRHL2, green; nuclei, magenta) and β-gal antibody (R–T: GRHL3, magenta; nuclei, blue) in wild-type (O–Q) and Grhl3^cre-nZ/+ (R-a). (U–X) β-gal (GRHL3; green) and GRHL2 (magenta) merged with DAPI staining (nuclei, blue). (Y-a) DKK1 (magenta; dotted lines), β-gal (GRHL3, green; arrowheads) and nuclei (DAPI, blue). Scale bars: 300 μm in A–E, H–L; 100 μm in F, G, M–V, Y-a; 10 μm in X.