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. Author manuscript; available in PMC: 2015 Aug 13.
Published in final edited form as: Circ Res. 2009 Apr 16;104(10):1201–1208. doi: 10.1161/CIRCRESAHA.109.195941

Figure 2.

Figure 2

Generation of transgenic mice with temporal and myocardial-restricted FRNK expression. a, Schematic of construct used to generate FRNKLoxP transgenic mice. b and c, Western blot analysis of myc-FRNK expression in tissues from FRNKMlc2v double transgenic mice. d, Immunohistochemical analysis of GFP expression levels in left ventricle from 8-week-old FRNKLoxP or FRNKMlc2v mice. e, Western analysis for active, phospho-Y397 FAK, or total FAK in ventricular lysate from 1-week FRNKLoxP or FRNKMlc2v mice. f, Viable FRNKNkx2.5 or FRNKMlc2v offspring at indicated time points. Dashed line denotes expected Mendelian ratio of 25%. See Online Table I for number of animals per time point. g, Western blot analysis of myc-FRNK expression in hearts of FRNKMlc2v and FRNKNkx2.5 double transgenic mice at the indicated ages. Western blots are representative of at least 3 separate experiments.