Figure 3. Capture of NifEN-B proteins in different redox states on affinity (IMAC) resin after incubation with [methyl-¹⁴C] SAM.

NifEN-B samples were as following: (1) reduced by excess dithionite; (2) reduced by dithionite, followed by removal of excess dithionite; (3) oxidized by IDS; (4) oxidized by IDS and then re-reduced by dithiothreitol; and (5) oxidized by IDS and then re-reduced by dithionite.