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Immunoblot analysis of interacting proteins purified from complexes from all E2 proteins.
E2 protein complexes were purified as described in Materials and Methods and Figure 3. The E2 complexes were eluted with 2% sarcosyl (HA bound) and analyzed by immunoblot analysis using various antibodies against components of the E2 protein complexes - E2 proteins (HA), BRD4, BAZ1B, NPM1, C1QBP, SRPK1, SRPK2, EFTUD2, and EIF4A3
