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RNase treatment of E2 complexes
FLAG-tagged HPV8 E2 protein was expressed in C-33A cells and purified with anti-FLAG M2 agarose without adding RNase A (fh and 8E2) or in presence of RNase A (fh-R and 8E2-R). The purified E2 complexes (FLAG eluates) were analyzed by immunoblot analysis using various antibodies against components of the E2 protein complexes - E2 proteins (FLAG), EFTUD2, SRPK2, EIF4A3, NPM1, and C1QBP.
