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. Author manuscript; available in PMC: 2015 Aug 13.
Published in final edited form as: J Am Chem Soc. 2009 Mar 11;131(9):3260–3270. doi: 10.1021/ja807969a

Figure 2.

Figure 2

Resonance Raman spectra of the ferric derivative of the H76S and H76A mutants of hTDO, with respect to the wild type enzyme, in the presence and absence of L-Trp. The small shoulder at 1357 cm−1 in the L-Trp bound wild type and H76S mutant is a result of a small amount of photoinduced reduction of the ferric heme. The protein concentrations are ~25–40 μM. The protein solutions were buffered at pH 7 with 100 mM phosphate. The concentration of L-Trp was 20 mM. The excitation wavelength for the Raman measurements was 413.1 nm and the laser power was ~5 mW. The sharp peaks labeled with asterisks are the plasma lines from the laser.