. Author manuscript; available in PMC: 2015 Aug 13.

Published in final edited form as: Biochemistry. 2011 Apr 25;50(19):3946–3956. doi: 10.1021/bi101137n

Table 1.

ν_Fe–O₂, ν_O–O, ν_Fe–CO, and ν_C–O Modes Determined by Resonance Raman Spectroscopy, O₂ Dissociation and Association Rate Constants (k_off and k_on), and O₂ Affinities (K_O₂), Calculated from k_on/k_off, of the Wild Type and Mutants of Ctb, Mt-trHbN, Mt-trHbO, and swMb

species	protein	ν_Fe–O₂ (cm⁻¹)	ν_O–O (cm⁻¹)	ν_Fe–CO (cm⁻¹)	ν_C–O (cm⁻¹)	k_on (μM⁻¹ s⁻¹)	k_off (s⁻¹)	K_O₂ (μM⁻¹)	ref
Ctb (trHbIII)	wt	542	1132	515	1936	0.91	0.0041	222	6
	E7_HL	550	1139	517, 557	1938, 1891	0.49	0.0003	1885	6
	B10_YF	552	1139	524	1914	0.12	0.0027	44	6
	B10_YF/E7_HL	557	1144	521	1926	42	0.0028	15 000	6
	G8_WF	552	1139	499, 548	1963, 1898	1.7	0.033	52	this work
Mt-trHbO (trHbII)	wt	559	1140	525	1914	0.13	0.0014 0.0058	93 22	4, 26, 54
Mt-trHbN (trHbI)	wt	562	1139	500, 535	1916, 1960	71	0.16	444	4, 13, 63
swMb	wt	569		508	1947	17	15	1.1	1, 67