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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 8;1839(11):1132–1140. doi: 10.1016/j.bbagrm.2014.09.003

Figure 3.

Figure 3

SRSF2 promotes splicing of intron 10 as well as intron 11. (A) RT-PCR analysis of endogenous splicing of intron 10 (left panel) and 11 (right panel) with RNAs extracted from non-treated cells, non-silencing shRNA virus treated cells and SRSF2 shRNA virus treated cells are shown. The primer sets used to detect intron 10 (1 and 2) and intron 11 (3 and 4) RT-PCR are shown. Quantitation results are shown at lower panel. (B) RT-PCR analysis of exogenous intron 10 and intron 11 with RNAs from pcDNA-SRSF2 overexpressed, pcDNA overexpressed and non-treated MDA MB 231 cells are shown. The primer sets used to detect intron 10 (1 and 2) and intron 11 (3 and 4) RT-PCR are shown. (C) RT-PCR analysis of exogenous intron 10 and intron 11 with RNAs from SRSF2 overexpressed, pcDNA overexpressed and non-treated MDA MB 231 cells from minigenes containing exon 10-11 or exon 11-12 are shown.