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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 8;1839(11):1132–1140. doi: 10.1016/j.bbagrm.2014.09.003

Figure 4.

Figure 4

SRSF2 interact with exon 11 to promote exon 11 inclusion. (A) The potential SRSF2 binding sites on exon 11 (bold) and the mutated nucleotide sequences (underlined) are highlighted. RT-PCR analysis of wild type Ron minigene and SRSF2 mutant construct with RNAs from non-treated, pcDNA overexpressed and SRSF2 overexpressed MDA MB 231 cells are shown. (B) 5’ biotinylated RNA sequences of wild type (WT) and mutant (Mut-SRSF2) are shown. Following incubation of biotinylated RNA with his-SRSF2 protein, we separated SRSF2-RNA complex from free RNA using native polyacrylamide gel electrophoresis. The biotin-labeled RNA was detected using streptavidin-HRP conjugates.