Fig. 1.

Construction of the recombinant expression vector pINA1297/pro-TGase. The hp4d promoter is indicated as a black arrow; the XPR2 signal sequence is indicated by a white box; the terminator is indicated by a box filled with vertical lines; the genes for selecting in Y. lipolytica or E. coli are indicated by slashed arrows; the zeta elements are indicated as two white arrows. The pro-TGase gene is inserted between the Sfi I and BamH I sites. The linearization sites allowing to liberate the “yeast cassette” are indicated by small arrows