Table 3.

Primers used in this study

Primer	Sequence (5′-3′)
1296P1	CCCAAGCTTGCCAGCGGCGGCGACG (underlined bases correspond to Hind III site)
1296P2	CGGGGTACCTTACGACCAGCCCTGCTTCACCTC (underlined bases correspond to Kpn I site)
1297P1	TTGGGCCGTTCTGGCCGCCAGCGGCGGCGACG (underlined bases correspond to Sfi I site)
1297P2	CGCGGATCCTTACGACCAGCCCTGCTTCACCTC (underlined bases correspond to BamH I site)
7N160Q1	CCCCAGGAGACGCAAGCCGAGTTT (underlined bases encode Gln)
7N160Q2	GCGTGGCCTGCTGTTTTCCAGGTC
7N355Q1	CGGCAGTGGTCTGCCGGGTA (underlined bases encode Gln)
7N355Q2	GAACTTGCTCTCGTATACGTTCATGGG