Table 1.
Sequences targeted by the shRNA expression plasmids used
| shRNA | Target sequence |
|---|---|
| shControl | TGGTTTACATGTCGACTAA |
| shEAP20-1 | CGTCAAGCTACAGCGAAAG |
| shEAP20-2 | CCTCGAGTGGTTGGATAAG |
| shEAP30-6 | GGAACTACATCAACAGGTG |
| shEAP30-7 | CCAGGATGTCAGTCAAGAT |
| shEAP30-8 | ACCTGATCAGAGCCATCAA |
| shEAP45-1 | GAATAAGGGCTGTAGGAAT |
| shEAP45-2 | TGATCAAGGCTAAGGAAAT |
The target sequence for shControl was derived from Dharmacon ON-TARGETplus siRNA. The remaining were selected using Clontech RNAi Target Sequence Selector (http://bioinfo.clontech.com/rnaidesigner/sirnaSequenceDesignInit.do) and MWG Biotech websites (http://www.eurofinsdna.com/products-services/sirna/sirna-design.html).