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. Author manuscript; available in PMC: 2015 Aug 13.
Published in final edited form as: J Invest Dermatol. 2013 May 23;133(12):2722–2731. doi: 10.1038/jid.2013.232

Figure 4. Adhesion and migration of β1 Y-to-A keratinocytes.

Figure 4

(a) Epidermis-derived single cell suspensions of normal and β1 Y-to-A epidermis were assayed for β1 integrin surface expression (mean ± SD; n=4; ***P<0.0001 vs. control). (b) Adhesion of normal and β1 Y-to-A keratinocytes to fibronectin and collagen coated tissue culture plastic over the indicated time period. (c) Spreading of normal and β1 Y-to-A keratinocytes on fibronectin and collagen coated tissue culture plastic (mean ± SD; n=3; *P<0.05 vs. control). (d) Scratch wounding of a confluent monolayer of normal and β1 Y795A keratinocytes. (e) Quantification of cell front migration into a scratch wound (mean ± SD; n=3; *P<0.05, ***P<0.0001 vs. control). (f) Assessment of migratory directionality. (g) Quantification of keratinocyte migratory velocity and accumulated distance (mean ± SD; n=3; *P<0.05 vs. control).