Figure 2. Mtb ΔglpX grows on gluconeogenic carbon sources and has detectable FBPase activity.
(a) Growth of WT Mtb (black), ΔglpX (red) and complemented strain ΔglpX/C (grey) in Sauton's minimal media containing no carbon source, 0.4% glucose, 0.1% glycerol or 0.1% butyrate. Data are representative of three independent experiments. (b) FBPase activity of WT Mtb (black), ΔglpX (red) and complemented strain ΔglpX/C (grey) cell lysates in the absence or presence of lithium chloride using 1.5 mM FBP or 12 mM FBP as substrate. Dashed line indicates limit of detection. Data are mean±s.d. of three biological replicates.