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. 2015 Aug 10;6:7912. doi: 10.1038/ncomms8912

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(a) Growth of WT Mtb (black), ΔglpX (red), ΔglpXΔgpm2 (orange) and complemented strains ΔglpXΔgpm2/C (glpX) (purple) and ΔglpXΔgpm2/C (gpm2) (blue) in Sauton's minimal media containing no carbon source, 0.4% glucose, 0.1% glycerol or 0.1% butyrate. Data are representative of three independent experiments. (b) FBPase activity of WT Mtb (black), ΔglpX (red), ΔglpXΔgpm2 (orange) and complemented strains ΔglpXΔgpm2/C (glpX) (purple) and ΔglpXΔgpm2/C (gpm2) (blue) cell lysates in the absence or presence of lithium chloride using 1.5 mM FBP or 12 mM FBP as substrate. ND indicates no detectable FBPase activity. Dashed line indicates limit of detection. Data are mean±s.d. of three biological replicates.