Table 1. Comparison of GPM2 and GLPX FBPase activity properties.
GPM2 | GLPX | |
---|---|---|
Enzymatic kinetics model | Allosteric sigmoidal | Michaelis–Menten |
Km | 5.51 mM | 44 μM |
kcat | 1.87 × 102 s−1 | 1.0 s−1 |
h | 3.02 | — |
kcat/Km | 3.39 × 104 s−1 M−1 | 2.27 × 104 s−1 M−1 |
FBPase, fructose bisphosphatase.
GPM2 enzymology determined experimentally using recombinant His-tagged GPM2. GLPX enzymology from published literature23.