Fig. 2.

Bcl2 inhibits mtDNA repair in association with increased frequency of mtDNA mutations. a. Levels of Bcl2 and APE1 were analyzed by Western blot in H1299 cells expressing WT Bcl2 and vector-only control. b and c. H1299 cells expressing WT Bcl2 and vector-only control were treated with 100 μM H₂O₂ (b) and 200 μM NNK (c) for 60 min. Cells were then washed three times and incubated in fresh culture medium for various times as indicated. mtDNA damage or mtDNA mutation was analyzed as described in “Methods”. Quantification data are mean ± SD from three independent experiments