Figure 13.

The NF-κB signaling pathway was regulated via ERK1/2 when THP-1-derived macrophages were treated with the novel NP-siRNA liposomes at 12.5 μg/mL for 24 hours.

Notes: The proteomic response was determined by SILAC, and pathways were analyzed using IPA. Red indicates an upregulation; green indicates a downregulation. The intensity of green and red colors indicates the degree of down- or upregulation. Solid arrow indicates direct interaction.

Abbreviations: ERK, extracellular signal-regulated kinase; IPA, ingenuity pathway analysis; NF-κB, nuclear factor-κB; NP, nanoparticle; SILAC, stable isotope labeling with amino acids in cell culture; siRNA, small interfering RNA.