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Construction of barcoded libraries. (A) Schematic of library construction. (B) Target regions in human TP53. The cDNA structure of the coding region of the human TP53 gene is shown via a bar graph of the distribution of mutations found in COSMIC v63 (http://cancer.sanger.ac.uk/). The dotted lines indicate the boundaries of exons in the DNA-binding domain. The black bars indicate target regions defined in this study.
