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Validation of microarray data by quantitative RT-PCR analysis on FLC expression in different mutants as compared to wild-type (set as 1).
| Mutant strain | Fold change in microarray | Relative level in RT-PCR |
|---|---|---|
| sdg25 | − 5.5 | 0.15 ± 0.02 |
| sdg26 | 2.4 | 3.24 ± 0.31 |
| sdg25 sdg26 | < 2.0 | 1.07 ± 0.15 |
| atx1 | − 6.0 | 0.24 ± 0.04 |
| sdg26 atx1 | < 2.0 | 1.08 ± 0.16 |
| clf | 3.8 | 3.80 ± 0.20 |
| sdg26 clf | 6.7 | 7.52 ± 0.11 |
| ldl1 ldl2 | < 2.0 | 1.92 ± 0.23 |
| sdg25 ldl1 ldl2 | < 2.0 | 1.17 ± 0.03 |
| sdg26 ldl1 ldl2 | 5.1 | 4.57 ± 0.62 |
