Additional file 12: Video S4.

Download video file ^{(1.1MB, mov)}

Video clip shows the transition through optical slices of a MCF10.DCIS structure in co-culture with CAF40TKi fibroblasts (Full 3D image shown in Fig. 4e and f). Cultures were fixed and immunostained for IL-6 (red), with DAPI labeled nuclei. MCF10.DCIS cells formed large nodular-like structures that branched out towards CFSE-labeled CAFs (pseudo-colored white). Images were obtained on a Zeiss LSM 780 and converted to a movie file using Volocity® software. (MOV 1 mb)