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. Author manuscript; available in PMC: 2015 Aug 13.
Published in final edited form as: Food Chem. 2011 Sep 6;131(2):387–396. doi: 10.1016/j.foodchem.2011.08.066

Figure 3. Effect of MB anthocyanins on glucose production and gene expression of G6P in H4IIE cells.

Figure 3

(A) Glucose production was measured in H4IIE cells after 8 h of treatment with ANC at doses of 0−40 μg/mL or metformin as a positive control at 2mM. Data represent the mean of three experiments +/− SD. (B) GP6ase mRNA expression was evaluated in H4IIE cells treated with Dex-cAMP (except for negative control) after 8 h of treatment with ANC at 50 μg/mL or 20 nM insulin. (C) An insulin-sensitising effect over G6P was evaluated in H4IIE cells treated with Dex-cAMP (except for negative control) after 8 h of treatment with ANC at 20 μg/mL together with insulin at 1 nM relative to insulin alone at 1 nM. G6P was normalised to β-actin mRNA and the specific fold changes were calculated as a ratio of the target mRNA level relative to the response to Dex-cAMP activation. In A, B and C data represent the mean of three experiments ± SD, **: p < 0.05.