Table 1.
Oligonucleotide primers used for the real-time PCR assays, the DNA walking approaches and the PCR confirmation of the transgenic junctions
| Methods | Oligonucleotide names | Oligonucleotide sequences | Product sizes (bp) | References |
|---|---|---|---|---|
| SYBR®Green qPCR | p35S F | AAAGCAAGTGGATTGATGTGATA | 75 | [6] |
| p35S R | GGGTCTTGCGAAGGATAGTG | [6] | ||
| SYBR®Green qPCR | tNOS F | GATTAGAGTCCCGCAATTATACATTTAA | 69 | [6] |
| tNOS R | TTATCCTAGKTTGCGCGCTATATTT | [6] | ||
| SYBR®Green qPCR | t35S pCAMBIA c-F | CGGGGGATCTGGATTTTAGTA | 137 | [14] |
| t35S pCAMBIA a-R | AGGGTTCCTATAGGGTTTCGCTC | [14] | ||
| DNA Walking | p35S-F a (p35S R) | GGGTCTTGCGAAGGATAGTG | [6] | |
| p35S-F b | TGTGCGTCATCCCTTACGTCAGT | / | This study | |
| p35S-F c | TATCACATCAATCCACTTGCTTT | [6] | ||
| DNA Walking | p35S-R a (p35S F) | AAAGCAAGTGGATTGATGTGATA | [6] | |
| p35S-R b | ACTGACGTAAGGGATGACGCACA | / | This study | |
| p35S-R c | CACTATCCTTCGCAAGACCC | [6] | ||
| DNA Walking | tNOS-F a (tNOS F) | GATTAGAGTCCCGCAATTATACATTTAA | [6] | |
| tNOS-F b | TTAATACGCGATAGAAAACAAAAT | / | This study | |
| tNOS-F c | AAATATAGCGCGCAAMCTAGGATAA | [6] | ||
| DNA Walking | tNOS-R a (tNOS R) | TTATCCTAGKTTGCGCGCTATATTT | [6] | |
| tNOS-R b | ATTTTGTTTTCTATCGCGTATTAA | / | This study | |
| tNOS-R c | TTAAATGTATAATTGCGGGACTCTAATC | [6] | ||
| PCR junction | Rice chromosome II | CCCCTAATTTCTCACAGGCC | 848 | This study |
| tNOS-F c | AAATATAGCGCGCAAMCTAGGATAA | [6] | ||
| PCR junction | Rice chromosome III | AGGTACTCAAGCCTTTTCCAGC | 1105 | This study |
| tNOS-F c | AAATATAGCGCGCAAMCTAGGATAA | [6] |