Skip to main content
. 2015 Aug 13;10(8):e0135033. doi: 10.1371/journal.pone.0135033

Fig 2. Localization of AtH1.1 and AtHMGB1 at different cell stages with or without paraformaldehyde fixation.

Fig 2

EGFP signals of living unfixed (columns 1–2) and fixed (columns 3–6) transgenic tobacco BY-2 cells were detected using confocal microscopy. AtH1.1-EGFP interacted with chromosome at different cell stages with or without fixation (rows 1–4). AtHMGB1-EGFP in living cells interacted with chromosomes at different cell stages captured (rows 5–8). However the interaction between AtHMGB1-EGFP with mitotic chromosomes was abolished when the cells were fixed with paraformaldehyde. White arrow heads indicate the position of the metaphase chromosomes. Only one typical image of each cell stage was shown. Additional images can be found in S2–S5 Figs. A total of 10, 8, 9 and 6 metaphase chromosomes images of unfixed AtHMGB1, unfixed AtH1.1, fixed AtHMGB1 and fixed AtH1.1 were taken, respectively.