Fig 3. Localization of AtH1.1 and AtHMGB1 at different cell stages detected by immunolabeling.

DAPI staining of chromosome (column 1), EGFP fluorescence signals (column 2), Rhodamine Red-X fluorescence signals (column 3) and DIC image (column 4) of transgenic tobacco BY-2 cells were detected using confocal microscopy. AtH1.1-EGFP interacted with chromosome at different cell stages with fixation (rows 1–3). The interaction between AtHMGB1-EGFP with mitotic chromosomes cannot be detected by both EGFP fluorescence and Rhodamine Red-X immunolabeled fluorescence upon fixation (rows 4–6). White arrow heads indicate the position of the metaphase chromosomes. Only one typical image of each cell stage was shown. Additional images can be found in S6 and S7 Figs. Eight images each of the metaphase chromosomes using AtHMGB1 or AtH1.1 antibodies were taken.