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. 2015 Aug 13;21:28. doi: 10.1186/s40409-015-0027-6

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© Menaldo et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a Chromatographic profile of fraction 6 on a C18 reverse phase column. Elution was performed using a RP-HPLC system at a flow rate of 0.5 mL/minute using a segmented concentration gradient of 0-60 % solvent B in three column volumes, 60-80 % in five column volumes and 80-100 % in one column volume. b 12 % SDS-PAGE. Lanes: 1 – PLA₂, 2 – molecular mass standard (116, 66.4, 45, 35, 25, 18.4, 14.4 kDa)