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. 2015 Aug 13;21:28. doi: 10.1186/s40409-015-0027-6

Fig. 5.

Fig. 5

a Evaluation of the purity level of fraction D4 after Vivaspin® 20 on a C18 reverse phase column. After having been subjected to ultrafiltration on a Vivaspin® 20 system, fraction D4 was evaluated by RP-HPLC using a C18 column with a linear concentration gradient of 0-100 % solvent B in five column volumes. b 12 % SDS-PAGE. Lanes: 1 – MP, 2 – molecular mass standard (260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa)