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. 2015 Aug 13;13(8):e1002213. doi: 10.1371/journal.pbio.1002213

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© 2015 Ander et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Circular dichroism spectra show a decrease for the nucleoprotein filament (left inset shows a magnified view). The dotted black line is a fit of arbitrary contributions of the spectra of ssDNA, dsDNA, free Redβ, Redβ +ssDNA, and Redβ +dsDNA such that the concurrently recorded absorbance spectra have a maximal overlap with that of the filament (see Fig C in S1 Text for details). In this manner, the decreased circular dichroism cannot be accounted for and, therefore, must stem from a structural change. The right inset shows a bathochromic circular dichroism shift between ssDNA, dsDNA, and filament.