Table 3. Comparison of NES from GSEA of GS defining distinct immune cell types (gene symbols described in S6 Table).

Cell type GS	n° genes per GS	NES:VHSV+	VHSVS	VHSVS+
NEUTROPHIL	16	1.27	0.61	0.81
MACROPHAGES	31	1.09	0.29	0.36
TH17	37	0.88	-0.8	0.54
DENDRITIC	10	0.64	-0.98	-1.1
BZ	23	0.62	-1.08	0.4
TH1	30	0.89	-1.18	0.4
TH2	31	0.93	-1.22	0.37
B	23	0.58	*-1.35	-0.84
NK CELLS	35	1.15	*-1.4	-0.84
TREG	25	0.88	*-1.54	-0.99
CTL	12	*-1.16	**-2.14	**-1.68

To estimate the different immune cell activities, new Gene Sets (GSs) were defined (gene compositions described in S6 Table). To define genes for each cellular type, activating, membrane and secreted genes were selected and added to the GS from data obtained from various sources. The resulting GSs shown by their symbols in the S6 Table, were used as inputs for GSEA analysis. The NES values of each cellular type ordered by those in VHSVS are shown. The differential expressions were calculated versus NI, non-infected zebrafish.

Th1, T helper 1 cells.

Th2, T helper 2 cells.

Th17, T helper 17 cells.

Treg, T regulatory cells.

B cells, IgM-producing cells.

BZ cells, IgZ-producing cells.

Dendritic, dendritic cells.

Cytotoxic, antigen-specific cytotoxic cells.

NK cells, natural killer cells.

Macrophages, monocyte and macrophages.

Neutrophil, neutrophil and granulocyte cells.

+, NES correlating with the first phenotype in the comparison.

-, NES correlating with NI in the comparison.

** (bold), FDR q value<0.05.

*, FDR q value <0.25.