Table 2. Methylation changes in C. bungeana subjected to the chilling (4°C) and freezing (-4°C) treatments for five periods of time (0–24 h).

		4°C				-4°C
		Methylation change		% changed		Methylation change		% changed
Group	marker× sample 1	(0>1) 2	(1>0) 2	Demethylation	Methylation	(0>1) 2	(1>0) 2	Demethylation	Methylation
0 h	80	6	2	7.5	2.5	6	2	7.5	2.5
0.5 h	80	25	9	31.25	11.25	27	11	33.75	13.75
3 h	80	34	15	42.5	18.75	29	22	36.25	27.5
12 h	80	26	9	32.5	11.25	20	19	25	23.75
24 h	80	27	13	33.75	16.25	16	13	20	16.25

In EcoRI/HpaII data, the presence (1) / absence (0) scores of 16 MS-AFLP loci at 4°C and -4°C are identified based on the consensus epigenotype at 0 h. At 4°C and -4°C, the changes in DNA methylation are inferred as either demethylation or methylation based on their divergence from the consensus epigenotype (0 h). The percentage of demethylation and methylation in the total 80 cases is also displayed.

¹, 16 MS-AFLP markers that were scored in each of five replicate plants for a total of 80 cases per group.

², Direction of the methylation change: 0>1, from absent to present; 1>0, from present to absent.