Table 4. Isolation and directional sequencing of MS-AFLP fragments.
The homologs of Arabidopsis genes were presented for functional description.
| Sequence (primer and position) | Length (bp) | Homologous sequence | E value | Alternation of DNA methylation pattern |
|---|---|---|---|---|
| E32H39_2–1 | 101 | Transposable element gene, AT2G04600 | 0.018 | Methylation |
| E36H41_2–2 | 120 | Encodes a protein that specifically binds plant Telomeric DNA repeats, AT5G13820 | 0.39 | Demethylation |
| E36H41_4–3 | 184 | UDP-glucosyltransferase 75B2, AT1G05560 | 0.87 | Demethylation |
| E37H39_3–1 | 95 | Regulator of Vps4 activity in the MVB pathway protein, AT4G32350 | 0.91 | Methylation |
| E38H40_2–2 | 226 | Xylose isomerase family protein, AT5G57655 | 0.42 | Demethylation |
| E45H39_2–3 | 173 | Encodes a mitochondrial glyoxalase 2, AT2G31350 | 3e-13 | Demethylation |
| E45H39_5–1 | 198 | P-loop containing nucleoside triphosphate hydrolases superfamily protein, AT5G37150 | 0.92 | Demethylation |
| E38H40_5–2 | 156 | Catalyzes the reduction of acetaldehyde using NADH as reductant. Alcohol dehydrogenase (NAD) activity, AT1G77120 | 2e-20 | Demethylation |
| E32-H39_1–1 & E32-H39_2–3 | 60 | Transposable element gene, AT1G36406 | 0.57 | Methylation |
| E45-H39_6–3 | 188 | Ulp1 protease family, AT4G07580 | 3.5 | Demethylation |
| E32-H39_1–2 | 125 | Unknown protein, AT1G25422 | 4e-06 | Demethylation |
| E32-H39_2–2 | 90 | Transducin/WD40 repeat-like superfamily protein, AT2G16405 | 0.96 | Methylation |
| E36-H41_1–1 | 195 | Unique among Arabidopsis Arm-repeat proteins, AT2G44900 | 0.079 | Demethylation |
| E36-H41_1–2 & E36-H41_2–2 & E36-H41_2–3 | 85&123&121 | Encodes a protein that specifically binds plant telomeric DNA repeats, AT5G13820 | 0.089&0.85&0.76 | Methylation |
| E36-H41_3–1 & E36-H41_3–2 | 206&208 | NagB/RpiA/CoA transferase-like superfamily protein, AT3G07300 | 3e-13 | Demethylation |
| E36-H41_4–1 | 171 | Unknown protein, AT5G14150 | 3.3 | Demethylation |
| E36-H41_4–3 | 184 | Polysaccharide biosynthetic process, AT3G57220 | 0.87 | Demethylation |
| E37-H39_1–1 | 116 | RUB1 activating enzyme, AT1G05180 | 0.98 | Demethylation |
| E37-H39_1–2 | 114 | ABC-2 and Plant PDR ABC-type transporter family protein, AT4G15233 | 3.8 | Methylation |
| E37-H39_2–1 | 123 | Mutator-like transposase family, AT3G31909 | 0.25 | Demethylation |
| E37-H39_2–2 | 122 | Unknown protein, AT5G61412 | 0.25 | Demethylation |
| E37-H39_3–1 | 195 | Regulator of Vps4 activity in the MVB pathway protein, AT4G32350 | 0.91 | Methylation |
| E37-H39_3–2 | 193 | Protein kinase superfamily protein, AT5G12090 | 0.90 | Methylation |
| E38-H40_1–1 | 160 | Gypsy-like retrotransposon family (Athila), AT5G33381 | 1e-06 | Methylation |
| E38-H40_1–2 | 158 | Gypsy-like retrotransposon family, AT5G29380 | 5e-09 | Methylation |
| E38-H40_1–3 | 105 | Disease resistance protein, AT5G41750 | 4e-06 | Demethylation |
| E38-H40_2–1 | 127 | Xylose isomerase family protein, AT5G57655 | 4.0 | Demethylation |
| E45-H39_1–3 | 197 | Unknown protein, AT4G17840 | 1e-15 | Demethylation |
| E45-H39_2–1 | 170 | Gypsy-like retrotransposon family, AT4G06620 | 8e-05 | Demethylation |
| E45-H39_2–2 | 170 | Encodes a mitochondrial glyoxalase 2, AT2G31350 | 2e-20 | Demethylation |
| E45-H39_3–1 | 145 | Encodes a protein containing ankyrin and DHHC-CRD domain, AT5G20350 | 4.2 | Demethylation |
| E45-H39_3–2 | 146 | F-box family protein, AT1G67130 | 0.27 | Demethylation |
| E45-H39_3–3 | 142 | ENTH/ANTH/VHS superfamily protein, AT1G03050 | 0.27 | Demethylation |
| E45-H39_4–1 | 129 | Unknown protein, AT5G20100 | 4.0 | Demethylation |
| E45-H39_4–2 | 135 | Unknown protein, AT5G37390 | 1e-15 | Demethylation |
| E45-H39_5–1 | 98 | P-loop containing nucleoside triphosphate hydrolases superfamily protein, AT5G37150 | 0.92 | Demethylation |
| E45-H39_6–1 | 93 | Gypsy-like retrotransposon family, AT5G34851 | 2e-04 | Methylation |
| E45-H39_6–2 | 92 | FAD-binding Berberine family protein, AT2G34810 | 3.6 | Methylation |
| E45-H41_3–3 | 166 | Polygalacturonase-inhibiting protein, AT5G06860 | 0.023 | Demethylation |
Methylation occurs if bands only appear in 0h rather than any other time of the cold treatment (0.5, 3, 12, 24h). However, demethylation occurs if the opposite is true.