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(A) A schematic diagram showing the pipeline of non-polyadenylated (poly(A)−/ribo−) RNA sequencing. (B) Validation of RPPH1 and UBB in R1 mouse embryonic stem cells (mESCs) by RT-PCR. (C) Classification of poly(A)+, poly(A)− and bimorphic predominant transcripts in mouse and rhesus monkey.
