Specifications
Organism	B6C3F1 mice
Sex	Female
Array type	Agilent SurePrint G3 Mouse GE 8x60K Microarray
Data format (in GEO)	Raw data: TXT files; normalized data: TXT files
Experimental factors	Furan exposed vs. un-exposed control
Experimental features	Female B6C3F1 mice were sub-chronically exposed for 21 days to control (0 mg/kg bw), non-carcinogenic (1, 2 mg/kg bw), and carcinogenic (4, 8 mg/kg bw) doses of furan. Half of the mice in each group were also given 0.02% BrdU for five days prior to sacrifice (days 16–21). All non-BrdU mice as well as the 0, 1, and 8 mg/kg bw furan + BrdU mice were used for gene expression analysis. Necropsy occurred four hours after the final furan dosing. RNA was extracted from livers and changes in gene expression were analyzed using Agilent microarrays.
Consent	All procedures were conducted in compliance with the Animal Welfare Act Regulations (9CFR1–4). Mice were handled and treated according to the guidelines provided in the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals (ILAR, 1996; http://dels.nas.edu/ilar/).
Sample source location	5–6 week old female specific pathogen free B6C3F1 mice were purchased from Charles River Breeding Laboratories (Portage, ME). Experiments were conducted at ILS, P.O. Box 13501, Research Triangle Park, NC 27709, USA.