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To analyze the dynamics of actin in the nucleus, an actin fusion protein, in which the actin was fused with the nuclear localization signal (NLS) and EYFP, was ectopically expressed in the HeLa cells (green). Filamentous actin (F-actin) was stained with Phalloidin-Alexa594 (red) and cellular DNA was stained with DAPI (blue). Scale bar = 10 μm.
