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. 2015 Aug 13;10(8):e0135315. doi: 10.1371/journal.pone.0135315

Fig 4. qRT-PCR analysis of WRKY gene expression during the overwintering.

Fig 4

C. heterophylla Fisch floral buds were collected each month from 2011 November to 2012 April. The ChActin gene was used as an internal control for qRT-PCR analysis. The relative expression (y-axis) was calculated using the 2−ΔΔCt formula. The mean value and standard error were obtained from three biological and three technical replicates.