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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Heart Lung Transplant. 2015 Mar 27;34(9):1154–1162. doi: 10.1016/j.healun.2015.03.021

Figure 6.

Figure 6

Knockdown of miR144 diminishes fibrogenesis in lung fibroblasts. (A) TaqMan RT-PCR analysis of miR-144 expression under 0, 6, 12, 24, and 36 hours treatment after TGF-β stimulation in lung fibrobalsts transfected with miR-144 inhibitor or control miR inhibitors. (B) Normal lung fibroblasts were transfected with 20 nM control inhibitors (con-anti-miR) or 20 nM inhibitors to miR-144 (anti-miR-144). Alpha-SMA and F-actin were chosen to evaluate fibrogenesis as the biomarkers. At 1 day after the transfection, cells were starved in medium containing 0.1% FBS for 1 day, followed by treatment without or with 2 ng/ml TGF-β for 1 d. Cells were then fixed and incubated with a mixture of anti-SMA-α antibody and FITC-conjugated phalloidin for staining of F-actin overnight. After washing 3 times, cells were incubated with Goat anti-mouse IgG H&L (Texas Red) for 1 h. Confocal microscopy was performed. Representative experiments are shown. Original view: ×63.