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. Author manuscript; available in PMC: 2016 Aug 10.
Published in final edited form as: Dev Cell. 2015 Jul 23;34(3):351–363. doi: 10.1016/j.devcel.2015.06.007

Figure 4. CCT1, but Not GPAT4, Falls Off a Shrinking Oil-Water Interface In Vitro.

Figure 4

(A) Schematic of the in vitro system. LDs in buffer are mixed with TG oil to generate a water-in-oil emulsion. LD proteins then bind to the resulting oil-water interface.

(B,C) During shrinkage of drops in vitro, CCT1 falls off the oil-water interface, whereas GPAT4 remains bound. (B) Representative images are shown. Scale bar, 10 μm. (C) Surface mean concentration and mean surface-bound fraction for mCherry-CCT1 and GFP-GPAT4 are reported. Lines represent trends. A.U. = arbitrary units. See also Figure S2.