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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Microbiol. 2015 Jun 6;97(4):746–758. doi: 10.1111/mmi.13061

Fig. 3. Permitting blocked forks to resume replication, resumes Mu repair.

Fig. 3

(A) The indicated ts mutants were infected with Mu after inactivation of replication at 42°C for 30 min (DnaEts) or 90 min (DnaCts). Following infection (0 min), cells held at 42°C (top row) were shifted down to 30°C (15 – 60 min, rows 2–4) to release the replication block, and monitored for presence of the FD. (B) Schematic depicting the time taken by the bidirectional fork originating at oriC to reach ter. Mu integrates all over the chromosome. On average, the fork would encounter Mu in ~20 min.