Table 1.
CUS, ketamine, HFS, and AST treatment schedule
Day 1 | Restraint (30 min) |
Day 2 | Shaking/crowding (1 hr; 6 rats/box; 220 back and forth movements/min) |
Day 3 | Social defeat (45 min continued protected exposure post-defeat) |
Day 4 | Warm swim ( 15 min) |
Day 5 | Wet bedding (24 hrs) |
Day 6 | Cold swim (10 min) |
Day7 | Shaking/crowding |
Day 8 | Footshock (15 min; 1.5mA, 5 sec on/ 5 sec off for 30 sec. every 3 min) |
Day 9 | Social defeat |
Day 10 | Warm swim (begin food restriction for AST) |
Day 11 | Footshock |
Day 12 | Tall pinch (10 min) |
Day 13 | Cold swim |
Day 14 | Footshock |
Day 15 | AST Habituation |
Day 16 | AST Training |
Day 17 | Drug injection or High frequency stimulation (HFS) |
Day 18 | AST Testing (24 hrs after HFS) |