Fig. 1.

ChIP-seq (a) and ChIP-qPCR (b) analysis of promoter and intragenic regions of Rpl13a was performed in CD4⁺ T cells cultured in the presence or absence of butyrate for 1 day, and immunoprecipitated using anti-acetyl histone H3 antibody (AcH3) or rabbit IgG as a negative control. Shading in Fig. 1a shows the ChIP-seq peaks. Orange boxes in Fig. 1a indicate the regions corresponding to the qPCR products in Fig. 1b. The error bars indicate standard deviation (n = 3). Comparison of peak height in Rpl13a and Gapdh genes in control cells (c). Normalized or raw peak height in Rpl13a genes in cells with or without butyrate (d).