Fig. 2.

ChIP-seq analysis of the promoter regions of all genes and transcription factors including Foxp3 with (red line) or without (blue line) normalization based on histone acetylation levels in Rpl13a loci. The distribution of acetylated H3 peak signals (a), green arrow indicates the application of Rpl13a-based normalization. Acetylated histone H3 levels in the Foxp3 gene locus with (−) or without (+) butyrate treatment (b).