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. Author manuscript; available in PMC: 2016 Aug 11.
Published in final edited form as: Cell Rep. 2015 Jul 30;12(6):1056–1068. doi: 10.1016/j.celrep.2015.07.019

Figure 3. Biophysical and biochemical characterization of Anc80L65.

Figure 3

A. Negative staining Transmission Electron Microscopy (TEM) of Anc80L65 particles. B. Anc80L65 viral protein composition by SDS PAGE: Purified AAV preparations were analyzed by SDS-PAGE demonstrating similar incorporation levels of monomers VP1, 2, and 3. C. Anc80L65 sedimentation and empty:full particle profile: Sedimentation coefficient distributions were derived from the refractive index optical measurement systems during analytical ultracentrifugation of AAV8 and Anc80L65 iodixanol purified preparations. D. Anc80L65 thermostability: SDS page thermostability assessment of AAV particle illustrates dissociation into monomers for AAV2 and 8 at temperatures below 70°C for AAV2 and 8 but requirin g 95°C melting temperatures for Anc80L65.