Figure 5.

Tail-length effects on solution degradation. Plots of V_max for steady-state degradation of a cp7-^SFGFP domain (A), a Halo domain (B), or a ^SFGFP domain (C) in substrates with a C-terminal ssrA tag (−) or an unfolded C-terminal V13P^CM-ssrA tag (+) by parental ClpXP and different p1-loop variants. Values are averages (N = 3) ± SEM. Initial rates of degradation of different concentrations of substrate by ClpX variants (0.1 μM), ClpP (0.3 μM), and ATP (4 mM) were determined and fit to the Michaelis-Menten equation to determine K_M and V_max values (Fig. S4) or were taken from Iosefson et al. (2015) (Table S1).