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. 2015 Aug 13;10(8):e0134566. doi: 10.1371/journal.pone.0134566

Fig 5. Six hours of regeneration.

Fig 5

A. SEM of the wound surface. B. SEM of wound surface covered by ECM layer. C. SEM of the apopyles with apopylar cells and choanocyte chamber remaining on the wound surface. D. Semithin section of wound surface with spread choanocyte chamber (scc). SEM (E) and semithin (F) section of single dedifferentiated choanocytes (arrowhead), that appeared on wound surfaces. G, H. TEM of choanocytes labeled with Indian ink (arrows) beginning of dedifferetntiation at the wound surface. ap—apopyle, b—symbiotic bacteria, cc—choanocyte chamber, enp—endopinacocytes, ECM—extracellular matrix, mv—microvilli, ph—phagosome, n—nucleus, scc—sectioned choanocyte chamber. Scale bars: A—25 μm, B, C, D—15 μm; E, F—5 μm, G—100 μm, H—50 μm.